The orbital Edelstein effect converts an electric field into a non-equilibrium orbital polarization, opening new opportunities for orbitronics. Although signatures of the orbital Edelstein effect have been reported, its microscopic mechanisms and quantitative validation remain underexplored. Here, by directly linking the atomic structure of KTaO₃(110) two-dimensional electron gases to both their calculated and measured electronic band dispersions, we predict and provide experimental evidence for an orbital Edelstein effect that largely counterbalances its spin counterpart. Scanning transmission electron microscopy and electron energy-loss spectroscopy resolve the interfacial atomic configuration, which is used as input for density-functional calculations. Angle-resolved photoemission spectroscopy then confirms the resulting band structure, which is fitted by a tight-binding model enabling computation of the spin and orbital Edelstein responses. Harmonic magnetotransport indicates that a ∼20 % contribution from the orbital Edelstein response is necessary to describe the magnitude and anisotropy of the effect. Our results establish KTaO₃(110) as a model platform for orbitronics and demonstrate a pathway to generate and harness orbital polarization in quantum oxide systems while also offering new insights into pairing mechanisms in their superconducting state.