Learning Gaussian mixture models (GMMs) using the Expectation-Maximization (EM) algorithm and its gradient-based variants is a fundamental problem in machine learning. It is known that randomly initialized (gradient) EM fails to learn multi-component GMMs in the exact-parameterized setting, where the number of components matches that of the ground-truth GMM. Recently, global convergence of gradient EM has been established in the over-parameterized setting, where more components are used, provided that the ground-truth components are well separated. In particular, the minimum separation between ground-truth components is required to scale as Ω(√d), where d is the dimension. In this paper, we show that this dimensional dependence is unavoidable in high-dimensional settings. Specifically, we consider a hybrid EM algorithm that uses standard EM updates for the mixing weights and gradient EM updates for the component means. For any ε > 0, we prove that when the dimension is sufficiently large, in the worst case a separation of order Ω(d^(0.5-ε)) is insufficient to guarantee global convergence of population gradient EM in sub-exponential time under random initialization, even in the over-parameterized regime. Our result establishes an almost optimal worst-case lower bound on the ground-truth separation required for learning Gaussian mixtures via gradient EM in high dimensions.